DNA quantification by real-time PCR in different forensic samples

Forensic Summary by Marissa Felinczak

The article DNA Quantification by Real-Time PCR in Different Forensic Samples discusses the quantification step in DNA analysis. The DNA analysis process includes many different steps. The process generally follows: extraction, quantification, amplification and normalization, and then analysis. The second step, quantification, is usually done by Real-Time Polymerase Chain Reaction or qPCR for short. Quantification is done in order to determine how much DNA was extracted from the source material.  It is done in order to provide validation to continue in the DNA analysis process—why continue through analysis if there is not enough DNA or no DNA? The qPCR instrument combines the functions of a thermal cycler and a fluorimeter. This means it has the ability to give a quantitative value for every sample unless there is not enough DNA to detect.

This study focuses on three different DNA source materials: blood, buccal swabs, and hair This study validated Quantifiler Trio DNA Quantification Kit as robust and accurate method for quantification. While different quantification kits are available, quantification is an important step in DNA analysis and is needed in the field of forensics for this purpose.

Martins, C. et al. DNA quantification by real-time PCR in different forensic samples. Forensic Science International: Genetics Supplement Series. 2015. doi:10.1016/j.fsigss.2015.09.215

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